The most severe mass extinction among animals took place in the late Permian (about 252 million years ago). The event was the largest extinction event with an estimated loss of ca. 90–96% of species. This included many kinds of fish, the last surviving trilobites and terrestrial vertebrates (including many of the large amphibians, mammal like reptiles and synapsids). It was also the largest known extinction of insects. Lystrosaurus was one of the few survivors.
Up to now it is still unclear what the mechanism for the extinction event was. There are various theories about the causes, these include: large or multiple meteorite impacts, increased volcanism (large flood basalt events like the Siberian Trap), and a sudden release of methane hydrates from the sea floor. Also a sea level change, oceanic anoxic events, increasing aridity, and a shift in ocean circulation driven by climate change are considered.